anti-eNOS antibody [M221]
anti-eNOS antibody [M221] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
|产品描述||Mouse Monoclonal antibody [M221] recognizes eNOS|
|反应物种||Hu, Ms, Rat|
|应用||FACS, ICC/IF, IHC-P, WB|
|抗原||Recombinant protein around the C-terminus region of Mouse eNOS. This sequence is conserved in Human and Rat eNOS, and has low homology to other NOS family members.|
|別名||Constitutive NOS; NOS type III; Nitric oxide synthase, endothelial; Endothelial NOS; eNOS; EC-NOS; NOSIII; cNOS; EC 184.108.40.206; ECNOS|
|应用说明||WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature.
IHC-P: Antigen Retrieval: Heat mediation was performed in 10 mM Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|缓冲液||PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA.|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|全名||nitric oxide synthase 3 (endothelial cell)|
|背景介绍||Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]|
|生物功能||Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. [UniProt]
|翻译后修饰||Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. [UniProt]|
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ARG23708 anti-eNOS antibody [M221] WB image
Western blot: Human umbilical vein endothelial cells untreated (left) or treated (right) with lambda phosphatase. The blots were stained with ARG23708 anti-eNOS antibody [M221].
ARG23708 anti-eNOS antibody [M221] ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed and NP-40-permeabilized Human umbilical vein endothelial cells. The cells were stained with ARG23708 anti-eNOS antibody [M221].