anti-eNOS antibody [M221]

anti-eNOS antibody [M221] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat


产品描述 Mouse Monoclonal antibody [M221] recognizes eNOS
反应物种 Hu, Ms, Rat
宿主 Mouse
克隆 Monoclonal
克隆号 M221
同位型 IgG1
靶点名称 eNOS
抗原物种 Mouse
抗原 Recombinant protein around the C-terminus region of Mouse eNOS. This sequence is conserved in Human and Rat eNOS, and has low homology to other NOS family members.
偶联标记 Un-conjugated
別名 Constitutive NOS; NOS type III; Nitric oxide synthase, endothelial; Endothelial NOS; eNOS; EC-NOS; NOSIII; cNOS; EC; ECNOS


应用 推荐稀释比
IHC-P1:500 - 1:1000
应用说明 WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature.
IHC-P: Antigen Retrieval: Heat mediation was performed in 10 mM Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


形式 Liquid
纯化 Protein A
缓冲液 PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA.
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 18127 Mouse NOS3

GeneID: 24600 Rat NOS3

GeneID: 4846 Human NOS3

基因名称 NOS3
全名 nitric oxide synthase 3 (endothelial cell)
背景介绍 Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
生物功能 Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. [UniProt]
预测分子量 133 kDa
翻译后修饰 Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. [UniProt]

检测图片 (2) Click the Picture to Zoom In

  • ARG23708 anti-eNOS antibody [M221] WB image

    Western blot: Human umbilical vein endothelial cells untreated (left) or treated (right) with lambda phosphatase. The blots were stained with ARG23708 anti-eNOS antibody [M221].

  • ARG23708 anti-eNOS antibody [M221] ICC/IF image

    Immunofluorescence: Paraformaldehyde-fixed and NP-40-permeabilized Human umbilical vein endothelial cells. The cells were stained with ARG23708 anti-eNOS antibody [M221].