anti-eNOS antibody

anti-eNOS antibody for IHC-Formalin-fixed paraffin-embedded sections and Human


产品描述 Mouse Monoclonal antibody recognizes eNOS
反应物种 Hu
应用 IHC-P
宿主 Mouse
克隆 Monoclonal
同位型 IgG
靶点名称 eNOS
抗原物种 Human
抗原 Purified recombinant fragment of Human eNOS.
偶联标记 Un-conjugated
別名 Constitutive NOS; NOS type III; Nitric oxide synthase, endothelial; Endothelial NOS; eNOS; EC-NOS; NOSIII; cNOS; EC; ECNOS


应用 推荐稀释比
IHC-P1:200 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


形式 Liquid
纯化 Affinity purified.
缓冲液 Ascitic fluid, 0.03% Sodium azide, 50% Glycerol and 0.5% BSA.
抗菌剂 0.03% Sodium azide
稳定剂 50% Glycerol and 0.5% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 4846 Human NOS3

Swiss-port # P29474 Human Nitric oxide synthase, endothelial

基因名称 NOS3
全名 nitric oxide synthase 3 (endothelial cell)
背景介绍 Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]
生物功能 Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

[Isoform eNOS13C]: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. [UniProt]
细胞定位 Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. [UniProt]
预测分子量 133 kDa
翻译后修饰 Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. [UniProt]

检测图片 (2) Click the Picture to Zoom In

  • ARG66856 anti-eNOS antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lymph node tissue stained with ARG66856 anti-eNOS antibody.

  • ARG66856 anti-eNOS antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon cancer tissue stained with ARG66856 anti-eNOS antibody.