ARG56831
anti-eIF6 antibody
anti-eIF6 antibody for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes eIF6 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | eIF6 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human eIF6. |
| 偶联标记 | Un-conjugated |
| 別名 | eIF-6; p27(BBP); b(2)gcn; p27BBP; EIF3A; Eukaryotic translation initiation factor 6; ITGB4BP; p27; 2; CAB; B4 integrin interactor; BBP |
应用说明
| 预测反应性说明 | Mouse, Rat | ||||||
|---|---|---|---|---|---|---|---|
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | K562 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | EIF6 |
| 全名 | eukaryotic translation initiation factor 6 |
| 背景介绍 | Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2012] |
| 生物功能 | Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity. [UniProt] |
| 预测分子量 | 27 kDa |
| 翻译后修饰 | Phosphorylation at Ser-174 and Ser-175 by CSNK1D/CK1 promotes nuclear export. |
检测图片 (2) Click the Picture to Zoom In
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ARG56831 anti-eIF6 antibody ICC/IF image
Immunofluorescence: MCF7 cells stained with ARG56831 anti-eIF6 antibody.
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ARG56831 anti-eIF6 antibody WB image
Western blot: K562 cell lysate stained with ARG56831 anti-eIF6 antibody.
