ARG58678
anti-eIF5B antibody
anti-eIF5B antibody for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes eIF5B |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | eIF5B |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-273 of Human EIF5B (NP_056988.3). |
| 偶联标记 | Un-conjugated |
| 別名 | Eukaryotic translation initiation factor 5B; Translation initiation factor IF-2; eIF-5B; IF2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa | ||||||
| 实际分子量 | 179 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | EIF5B |
| 全名 | eukaryotic translation initiation factor 5B |
| 背景介绍 | Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008] |
| 生物功能 | Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 139 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG58678 anti-eIF5B antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG58678 anti-eIF5B antibody at 1:100 dilution.
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ARG58678 anti-eIF5B antibody WB image
Western blot: 25 µg of HeLa cell lysate stained with ARG58678 anti-eIF5B antibody.
