ARG65003
anti-eIF5A antibody
anti-eIF5A antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes eIF5A |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 特异性 | Reported variants NP_001137233.1, NP_001961.1 and NP_001137234.1 represent identical protein: |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | eIF5A |
| 抗原物种 | Human |
| 抗原 | C-RPPHRASFLKRLESK |
| 偶联标记 | Un-conjugated |
| 別名 | eIF5AI; Eukaryotic translation initiation factor 5A-1; eIF-5A-1; Rev-binding factor; Eukaryotic initiation factor 5A isoform 1; eIF-5A1; EIF-5A; eIF-4D; EIF5A1; eIF-5A |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P63241 Human Eukaryotic translation initiation factor 5A-1 |
|---|---|
| 基因名称 | EIF5A |
| 全名 | eukaryotic translation initiation factor 5A |
| 生物功能 | mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts. [UniProt] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | Isoform 1: 17 kDa Isoform 2: 20 kDa |
| 翻译后修饰 | Acetylated. Deacetylated by SIRT2. eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine). |
检测图片 (4) Click the Picture to Zoom In
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ARG65003 anti-eIF5A antibody WB image
Western Blot: MOLT4 lysate (35 µg protein in RIPA buffer) stained with ARG65003 anti-eIF5A antibody at 0.1 µg/ml dilution.
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ARG65003 anti-eIF5A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65003 anti-eIF5A antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG65003 anti-eIF5A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65003 anti-eIF5A antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG65003 anti-eIF5A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human vessel tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65003 anti-eIF5A antibody at 3.75 µg/ml dilution followed by AP-staining.
