ARG58630
anti-eIF5A antibody
anti-eIF5A antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes eIF5A |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | eIF5A |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human eIF5A. |
| 偶联标记 | Un-conjugated |
| 別名 | eIF5AI; Eukaryotic translation initiation factor 5A-1; eIF-5A-1; Rev-binding factor; Eukaryotic initiation factor 5A isoform 1; eIF-5A1; EIF-5A; eIF-4D; EIF5A1; eIF-5A |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Jurkat | ||||||||||
| 实际分子量 | ~ 20 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | EIF5A |
| 全名 | eukaryotic translation initiation factor 5A |
| 生物功能 | mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts. [UniProt] |
| 细胞定位 | Nucleus > Nuclear pore complex. [UniProt] |
| 预测分子量 | Isoform 1: 17 kDa Isoform 2: 20 kDa |
| 翻译后修饰 | Acetylated. Deacetylated by SIRT2. eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine). [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG58630 anti-eIF5A antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG58630 anti-eIF5A antibody.
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ARG58630 anti-eIF5A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human uterus cancer stained with ARG58630 anti-eIF5A antibody.
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ARG58630 anti-eIF5A antibody WB image
Western blot: Jurkat cell lysate stained with ARG58630 anti-eIF5A antibody.
