ARG65706
anti-eIF4E antibody
anti-eIF4E antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes eIF4E |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | eIF4E |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human eIF4E |
| 偶联标记 | Un-conjugated |
| 別名 | EIF4E1; EIF4EL1; Eukaryotic translation initiation factor 4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; CBP; eIF-4E; eIF4E; AUTS19; EIF4F |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | 293T |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | EIF4E |
| 全名 | eukaryotic translation initiation factor 4E |
| 背景介绍 | The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| 生物功能 | Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. [UniProt] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. |
检测图片 (2) Click the Picture to Zoom In
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ARG65706 anti-eIF4E antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney stained with ARG65706 anti-eIF4E antibody at 1:200 dilution.
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ARG65706 anti-eIF4E antibody WB image
Western blot: 293T cell lysate stained with ARG65706 anti-eIF4E antibody.
