ARG51814
anti-eIF4B phospho (Ser422) antibody
anti-eIF4B phospho (Ser422) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes eIF4B phospho (Ser422) |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | eIF4B |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of Serine 422 (T-G-S(p)-E-S) derived from Human eIF4B. |
| 偶联标记 | Un-conjugated |
| 別名 | EIF-4B; eIF-4B; PRO1843; Eukaryotic translation initiation factor 4B |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P23588 Human Eukaryotic translation initiation factor 4B Swiss-port # Q8BGD9 Mouse Eukaryotic translation initiation factor 4B |
|---|---|
| 基因名称 | EIF4B |
| 全名 | eukaryotic translation initiation factor 4B |
| 背景介绍 | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. |
| 生物功能 | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. [UniProt] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 69 kDa |
| 翻译后修饰 | Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex. |
检测图片 (2) Click the Picture to Zoom In
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ARG51814 anti-eIF4B phospho (Ser422) antibody WB image
Western blot: Extracts from HeLa cells untreated or treated with EGF stained with ARG51814 anti-eIF4B phospho (Ser422) antibody.
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ARG51814 anti-eIF4B phospho (Ser422) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung carcinomatissue stained with ARG51814 anti-eIF4B phospho (Ser422) antibody (left) or the same antibody preincubated with blocking peptide (right).
