ARG57332

anti-eIF4A1 antibody

anti-eIF4A1 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes eIF4A1
反应物种 Hu, Ms, Rat
应用 ICC/IF, IP, WB
特异性 This antibody might also react to eIF4A2 based on sequence analysis (90%).
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 eIF4A1
抗原物种 Human
抗原 Recombinant Protein of Human eIF4A1.
偶联标记 Un-conjugated
別名 ATP-dependent RNA helicase eIF4A-1; EIF4A; Eukaryotic initiation factor 4A-I; EIF-4A; DDX2A; EC 3.6.4.13; eIF4A-I; eIF-4A-I

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:200
IP1:20 - 1:50
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 HeLa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 13681 Mouse EIF4A1

GeneID: 1973 Human EIF4A1

Swiss-port # P60842 Human Eukaryotic initiation factor 4A-I

Swiss-port # P60843 Mouse Eukaryotic initiation factor 4A-I

基因名称 EIF4A1
全名 eukaryotic translation initiation factor 4A1
生物功能 ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. [UniProt]
预测分子量 46 kDa

检测图片 (2) Click the Picture to Zoom In

  • ARG57332 anti-eIF4A1 antibody ICC/IF image

    Immunofluorescence: MCF-7 cells stained with ARG57332 anti-eIF4A1 antibody.

  • ARG57332 anti-eIF4A1 antibody WB image

    Western blot: HeLa cell lysate stained with ARG57332 anti-eIF4A1 antibody.