ARG42137
anti-eEF1E1 / AIMP3 antibody
anti-eEF1E1 / AIMP3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Pig
概述
| 产品描述 | Goat Polyclonal antibody recognizes eEF1E1 / AIMP3 |
|---|---|
| 反应物种 | Hu, Pig |
| 预测物种 | Cow, Dog |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | eEF1E1 / AIMP3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the internal region of Human eEF1E1 / AIMP3. (C-SKGNKYSAQGERQ) (NP_004271.1; NP_001129122.1) |
| 偶联标记 | Un-conjugated |
| 別名 | Eukaryotic translation elongation factor 1 epsilon-1; Elongation factor p18; AIMP3; P18; Multisynthase complex auxiliary component p18; Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||
| 阳性对照 | K562 and Pig testis | ||||||
| 实际分子量 | ~ 20 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O43324 Human Eukaryotic translation elongation factor 1 epsilon-1 |
|---|---|
| 基因名称 | EEF1E1 |
| 全名 | eukaryotic translation elongation factor 1 epsilon 1 |
| 背景介绍 | This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2010] |
| 生物功能 | Positive modulator of ATM response to DNA damage. [UniProt] |
| 细胞定位 | Cytoplasm. Cytoplasm, cytosol. Nucleus. Note=Cytoplasmic under growth arrest conditions. Translocated into the nucleus when growth resumes (S phase) and following DNA damage. [UniProt] |
| 预测分子量 | 20 kDa |
检测图片 (3) Click the Picture to Zoom In
-
ARG42137 anti-eEF1E1 / AIMP3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human pancreas tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42137 anti-eEF1E1 / AIMP3 antibody at 5 µg/ml dilution followed by AP-staining.
-
ARG42137 anti-eEF1E1 / AIMP3 antibody WB image
Western blot: 35 µg of K562 cell lysate (in RIPA buffer) stained with ARG42137 anti-eEF1E1 / AIMP3 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
-
ARG42137 anti-eEF1E1 / AIMP3 antibody WB image
Western blot: 35 µg of Pig testis lysate (in RIPA buffer) stained with ARG42137 anti-eEF1E1 / AIMP3 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
