ARG58646
anti-eEF1D antibody
anti-eEF1D antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes eEF1D |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | eEF1D |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-160 of Human eEF1D (NP_001123529.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Antigen NY-CO-4; FP1047; EF-1-delta; EF1D; EF-1D; Elongation factor 1-delta |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | A549 | ||||||||||
| 实际分子量 | 38 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | EEF1D |
| 全名 | eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) |
| 背景介绍 | This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010] |
| 生物功能 | Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome. Isoform 2: Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE). [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 31 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG58646 anti-eEF1D antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG58646 anti-eEF1D antibody.
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ARG58646 anti-eEF1D antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG58646 anti-eEF1D antibody at 1:200 dilution.
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ARG58646 anti-eEF1D antibody WB image
Western blot: 25 µg of A549 cell lysate stained with ARG58646 anti-eEF1D antibody at 1:1000 dilution.
