ARG52271
anti-eEF1A2 phospho (Ser358) antibody
anti-eEF1A2 phospho (Ser358) antibody for Western blot and Mouse
Cancer antibody; Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes eEF1A2 phospho (Ser358) |
|---|---|
| 反应物种 | Ms |
| 预测物种 | Hu, Rat, Chk, NHuPrm, Sheep, Xenopus laevis |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | eEF1A2 |
| 抗原物种 | Human |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | EF1A; Eukaryotic elongation factor 1 A-2; eEF1A-2; Elongation factor 1-alpha 2; STN; EIEE33; STNL; Statin-S1; HS1; EEF1AL; EF-1-alpha-2; MRD38 |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~50k eEF1A2 phosphorylated at Ser358. Immunolabeling of the eEF1A2 band is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | EEF1A2 |
| 全名 | eukaryotic translation elongation factor 1 alpha 2 |
| 背景介绍 | Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle (Knudsen et al., 1993). Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors (Anand et al., 2002). Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites (Soares et al., 2009). Ribosome-associated JNK phosphorylates Ser358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome (Gandin et al., 2013). |
| 研究领域 | Cancer antibody; Gene Regulation antibody |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Trimethylated at Lys-165 by EEF1AKMT3. |
检测图片 (1) Click the Picture to Zoom In
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ARG52271 anti-eEF1A2 phospho (Ser358) antibody WB image
Western blot: Mouse hippocampus lysate showing specific immunolabeling of the ~ 50k eEF1A2 protein phosphorylated at Ser 358 stained with ARG52271 anti-eEF1A2 phospho (Ser358) antibody.
Immunolabeling is blocked by treatment of the lysate with lambda phosphatase (P-tase).
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