ARG58639
anti-eEF1A1 + eEF1A2 antibody
anti-eEF1A1 + eEF1A2 antibody for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes eEF1A1 + eEF1A2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | eEF1A1 + eEF1A2 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 123-462 of Human eEF1A1 (NP_001393.1). |
| 偶联标记 | Un-conjugated |
| 別名 | EE1A1; EEF-1; Eukaryotic elongation factor 1 A-1; CCS-3; GRAF-1EF; LENG7; EEF1A; CCS3; HNGC:16303; EF-Tu; Elongation factor Tu; Leukocyte receptor cluster member 7; eEF1A-1; PTI1; EF1A; EF-1-alpha-1; Elongation factor 1-alpha 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa | ||||||
| 实际分子量 | 50 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | EEF1A1 |
| 全名 | eukaryotic translation elongation factor 1 alpha 1 |
| 背景介绍 | This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008] |
| 生物功能 | This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. [UniProt] |
| 细胞定位 | Cytoplasm, Nucleus, nucleolus. [UniProt] |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | ISGylated. Phosphorylated by TXK. Phosphorylation by PASK increases translation efficiency. Phosphorylated by ROCK2 (PubMed:26497934). Trimethylated at Lys-79 by EEF1AKMT1 (PubMed:26545399). Methylated at Lys-165 by EEF1AKMT3, methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation (PubMed:28108655). Trimethylated at Lys-318 by EEF1AKMT2 (PubMed:25144183). [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG58639 anti-eEF1A1 + eEF1A2 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG58639 anti-eEF1A1 + eEF1A2 antibody.
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ARG58639 anti-eEF1A1 + eEF1A2 antibody WB image
Western blot: 25 µg of HeLa cell lysate stained with ARG58639 anti-eEF1A1 + eEF1A2 antibody at 1:1000 dilution.
