ARG40572
anti-beta Tubulin antibody (HRP)
anti-beta Tubulin antibody (HRP) for Western blot and Human,Mouse,Rat,Hamster,Monkey
Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody
概述
| 产品描述 | HRP-conjugated Mouse Monoclonal antibody recognizes beta Tubulin |
|---|---|
| 反应物种 | Hu, Ms, Rat, Hm, Mk |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG1 |
| 靶点名称 | beta Tubulin |
| 抗原物种 | Human |
| 抗原 | Purified recombinant Human beta Tubulin protein fragments. |
| 偶联标记 | HRP |
| 別名 | OK/SW-cl.56; CDCBM6; Tubulin beta chain; M40; TUBB5; Tubulin beta-5 chain; TUBB1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 50% Glycerol and 0.1 mg/ml BSA. |
| 稳定剂 | 50% Glycerol and 0.1 mg/ml BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TUBB |
| 全名 | tubulin, beta class I |
| 背景介绍 | This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014] |
| 生物功能 | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProt] |
| 细胞定位 | Cytoplasm, cytoskeleton. [UniProt] |
| 研究领域 | Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG40572 anti-beta Tubulin antibody (HRP) WB image
Western blot: 3T3 cell lysate stained with ARG40572 anti-beta Tubulin antibody (HRP) at 1:10000 dilution.
