ARG62347
anti-beta Tubulin antibody [BT7R]
anti-beta Tubulin antibody [BT7R] for Dot blot,ELISA,ICC/IF,Western blot and Human,Mouse,Rat,Chicken,Monkey,Rabbit
Controls and Markers antibody; Signaling Transduction antibody
参考文献8
概述
| 产品描述 | Mouse Monoclonal antibody [BT7R] recognizes beta Tubulin |
|---|---|
| 反应物种 | Hu, Ms, Rat, Chk, Mk, Rb |
| 应用 | Dot, ELISA, ICC/IF, WB |
| 特异性 | Recognizes native and denatured forms of beta Tubulin (about 50 kDa). |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | BT7R |
| 同位型 | IgG2a |
| 靶点名称 | beta Tubulin |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide around the N-terminal region of Human beta Tubulin. |
| 偶联标记 | Un-conjugated |
| 別名 | Tubulin beta-1 chain |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Mouse brain tissue lysates |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | Protein A affinity chromatography from Mouse ascites fluid. |
| 缓冲液 | 10mM PBS (pH 7.2) and 0.05% Sodium azide |
| 抗菌剂 | 0.05% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TUBB1 |
| 全名 | tubulin, beta 1 class VI |
| 背景介绍 | Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y. [provided by RefSeq, Jul 2010] |
| 生物功能 | Tubulins is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProt] |
| 细胞定位 | Cytoplasm › cytoskeleton |
| 产品亮点 | Related Antibody Duos and Panels: ARG30330 Pyroptosis Antibody Panel Related products: beta Tubulin antibodies; beta Tubulin Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| 研究领域 | Controls and Markers antibody; Signaling Transduction antibody |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. |
检测图片 (4) Click the Picture to Zoom In
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ARG62347 anti-beta Tubulin antibody [BT7R] WB image
Western blot: Rat basolateral amygdala stained with ARG62347 anti-beta Tubulin antibody [BT7R] at 1:1000 dilution, ARG65350 Goat anti-Mouse IgG antibody (HRP) at 1:5000 dilution.
From Guang-Bing Duan et al. CNS Neurosci Ther. (2024), doi: 10.1111/cns.14611, Fig. 4.D.
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ARG62347 anti-beta Tubulin antibody [BT7R] WB image
Western blot: 20 μg of PC3, MCF7, MDA-MD-231, K562, M. brain, R. brain, MCF7, HT29, 3T3 and Raw264.7 cell lysates stained with ARG62347 anti-beta Tubulin antibody [BT7R] at 1:3000 dilution.
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ARG62347 anti-beta Tubulin antibody [BT7R] WB image
Western blot: 20 µg of 293T, Mouse brain and Rat brain lysates stained with ARG62347 anti-beta Tubulin antibody [BT7R] at 1:10000 dilution.
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ARG62347 anti-beta Tubulin antibody [BT7R] WB image
Western blot: U87-MG and SW480 cell lysates stained with ARG62347 anti-beta Tubulin antibody [BT7R] at 1:1000 dilution.
客户反馈
Excellent
anti-beta Tubulin antibody [BT7R]
Application：WB
Sample：293T, Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:10000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-beta Tubulin antibody [BT7R]
Application：WB
Sample：PC3, MCF7, MDA-MD-231, K562, M. brain, R. Brain, MCF7, HT29, 3T3, and Raw264.7
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:3000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-beta Tubulin antibody [BT7R]
Application：WB
Sample：K562, Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:4000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-beta Tubulin antibody [BT7R]
Application：WB
Sample：HEK293T, HepG2 and Jurkat cells
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:3000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
anti-beta Tubulin antibody [BT7R]
Application：WB
Sample：293T and HeLa cells
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:2000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
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Foxo1 selectively regulates static mechanical pain by interacting with Nav1.7.
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Protection against Doxorubicin-Induced Cardiotoxicity through Modulating iNOS/ARG 2 Balance by Electroacupuncture at PC6.
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ATF4 selectively regulates heat nociception and contributes to kinesin-mediated TRPM3 trafficking.
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