ARG11148
anti-beta Synuclein antibody [6A10]
anti-beta Synuclein antibody [6A10] for IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Pig
概述
| 产品描述 | Mouse Monoclonal antibody [6A10] recognizes beta Synuclein |
|---|---|
| 反应物种 | Hu, Ms, Rat, Cow, Pig |
| 应用 | IHC-Fr, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 6A10 |
| 同位型 | IgG1 |
| 靶点名称 | beta Synuclein |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide around the C-terminal region of Human beta Synuclein. (EPEGESYEDPPQEEYQEYEPEA) |
| 偶联标记 | Un-conjugated |
| 別名 | Beta-synuclein |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | ~ 18 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified |
| 缓冲液 | PBS, 5 mM Sodium azide and 50% Glycerol. |
| 抗菌剂 | 5 mM Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SNCB |
| 全名 | synuclein, beta |
| 背景介绍 | This gene encodes a member of a small family of proteins that inhibit phospholipase D2 and may function in neuronal plasticity. The encoded protein is abundant in lesions of patients with Alzheimer disease. A mutation in this gene was found in individuals with dementia with Lewy bodies. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] |
| 生物功能 | Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 14 kDa |
| 翻译后修饰 | Phosphorylated. Phosphorylation by G-protein coupled receptor kinases (GRK) is more efficient than phosphorylation by CK1, CK2 and CaM-kinase II. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG11148 anti-beta Synuclein antibody [6A10] IHC-Fr image
Immunohistochemistry: Frozen section of Mouse hippocampus tissue stained with ARG11148 anti-beta Synuclein antibody [6A10] (green) at 1:500 dilution, and co-stained with ARG11140 anti-Neurofilament NF-L (C-ter) antibody (red) at 1:5000 dilution. Hoechst (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.).
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ARG11148 anti-beta Synuclein antibody [6A10] WB image
Western blot: Rat cortex, Rat cerebellum, Mouse cortex, Mouse cerebellum, Cow cortex and Cow cerebellum lysates stained with ARG11148 anti-beta Synuclein antibody [6A10] at 1:1000 dilution.
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ARG11148 anti-beta Synuclein antibody [6A10] IHC-Fr image
Immunohistochemistry: Frozen section of Rat cerebellum tissue stained with ARG11148 anti-beta Synuclein antibody [6A10] (green) at 1:500 dilution, and co-stained with ARG11111 anti-Calbindin antibody (red) at 1:5000 dilution. Hoechst (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.).
