ARG54472

anti-beta Lactamase antibody [8A5.A10]

Datasheet

订购信息

包装

价格 (USD)

200 μg

399

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Mouse Monoclonal antibody [8A5.A10] recognizes beta Lactamase
反应物种	Bacteria
应用	ELISA
特异性	This antibody recognizes TEM-1-type beta-lactamases.
宿主	Mouse
克隆	Monoclonal
克隆号	8A5.A10
同位型	IgG1
靶点名称	beta Lactamase
抗原物种	E. coli
抗原	5’-His-tagged E. coli 205 TEM-1 R+ beta-lactamase, accession no. P62593. Sequence: MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW
偶联标记	Un-conjugated

应用说明

应用说明	Western blot: use at 10 ug/ml. Predicted molecular weight ~29kDa. ELISA: use at 10 - 20 ug/ml (optimized for beta - lactamase on solid phase at 10 ug/ml). These are recommended concentrations. Enduser should determine optimal concentrations for their application. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Western blot: use at 10 ug/ml. Predicted molecular weight ~29kDa. ELISA: use at 10 - 20 ug/ml (optimized for beta - lactamase on solid phase at 10 ug/ml). These are recommended concentrations. Enduser should determine optimal concentrations for their application.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Protein G-purified
缓冲液	PBS (pH 7.4)
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

背景介绍	The beta-lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta-lactams are structurally related through the presence of a core beta-lactam ring. Bacterial resistance to beta-lactams continues to increase, primarily due to microbial production of beta-lactamases. Beta-lactamases catalyze the hydrolysis of the beta-lactam bond which destroys anti-bacterial activity. Bacteria the produce TEM- or SHV-type beta-lactamases have point mutations in structural genes that have extended the substrate specificity of these betalactamases. As a result, many of the beta-lactamase-producing Gram-negative bacteria have become multi-drug resistant.
研究领域	Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量	32 kDa

克隆号文献

Antibody-mediated immunity induced by engineered Escherichia coli OMVs carrying heterologous antigens in their lumen.

WB /

Fantappiè L et al.
J Extracell Vesicles., (2014)

Unique substrates secreted by the type VI secretion system of Francisella tularensis during intramacrophage infection.

WB /

Bröms JE et al.
PLoS One., (2012)

Combinatorial mutagenesis and selection of improved signal sequences and their application for high-level production of translocated heterologous proteins in Escherichia coli.

WB /

Heggeset TM et al.
Appl Environ Microbiol., (2013)

Comprehensive identification of protein substrates of the Dot/Icm type IV transporter of Legionella pneumophila.

WB /

Zhu W et al.
PLoS One., (2011)

Versatile selection technology for intracellular protein-protein interactions mediated by a unique bacterial hitchhiker transport mechanism.

WB / Escherichia coli

Waraho D et al.
Proc Natl Acad Sci U S A., (2009)