ARG44762
anti-beta III Tubulin antibody
anti-beta III Tubulin antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse
概述
| 产品描述 | Mouse Monoclonal antibody recognizes beta III Tubulin |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2b |
| 靶点名称 | beta III Tubulin |
| 抗原物种 | Human |
| 偶联标记 | Un-conjugated |
| 別名 | CDCBM1; Tubulin beta-4 chain; Tubulin beta-3 chain; CFEOM3A; Tubulin beta-III; TUBB4; CDCBM; CFEOM3; FEOM3; beta-4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein A purification |
| 缓冲液 | PBS with 0.09% sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TUBB3 |
| 全名 | tubulin, beta 3 class III |
| 背景介绍 | This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010] |
| 生物功能 | In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. [UniProt] |
| 细胞定位 | Cytoplasm, cytoskeleton. [UniProt] |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG44762 anti-beta III Tubulin antibody IHC-P image
Immunohistochemistry: Human cerebellum stained with ARG44762 anti-beta III Tubulin antibody at 5 µg/mL dilution.
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ARG44762 anti-beta III Tubulin antibody WB image
Western blot: MCF7 and SH-SY5Y stained with ARG44762 anti-beta III Tubulin antibody at 1 µg/mL dilution.
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ARG44762 anti-beta III Tubulin antibody IP image
Immunoprecipitation: MCF7 lysate immunoprecipitated with 2.5 µg of ARG44762 anti-beta III Tubulin antibody.
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ARG44762 anti-beta Tubulin III antibody WB image
Western blot: Mouse brain stained with ARG44762 anti-beta Tubulin III antibody at 1 µg/mL dilution.
