ARG42915
anti-beta III Tubulin antibody
anti-beta III Tubulin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes beta III Tubulin |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | beta III Tubulin |
| 抗原物种 | Human |
| 抗原 | Human beta III Tubulin. |
| 偶联标记 | Un-conjugated |
| 別名 | CDCBM1; Tubulin beta-4 chain; Tubulin beta-3 chain; CFEOM3A; Tubulin beta-III; TUBB4; CDCBM; CFEOM3; FEOM3; beta-4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | SK-N-SH and Mouse brain | ||||||||
| 实际分子量 | ~ 50 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300, 20% Glycerol and 1% BSA. |
| 抗菌剂 | 0.025% ProClin 300 |
| 稳定剂 | 20% Glycerol and 1% BSA |
| 浓度 | 0.57 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TUBB3 |
| 全名 | tubulin, beta 3 class III |
| 背景介绍 | This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010] |
| 生物功能 | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977). [UniProt] |
| 细胞定位 | Cytoplasm, cytoskeleton. [UniProt] |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG42915 anti-beta III Tubulin antibody ICC/IF image
Immunofluorescence: HUVEC cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with ARG42915 anti-beta III Tubulin antibody (red) at 1:200 dilution and 4°C. DAPI (blue) for nuclear staining.
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ARG42915 anti-beta III Tubulin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human gastric carcinoma tissue stained with ARG42915 anti-beta III Tubulin antibody at 1:100 dilution.
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ARG42915 anti-beta III Tubulin antibody WB image
Western blot: 20 µg of SK-N-SH cell lysate stained with ARG42915 anti-beta III Tubulin antibody at 1:5000 dilution.
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ARG42915 anti-beta III Tubulin antibody WB image
Western blot: 20 µg of Mouse brain lysate stained with ARG42915 anti-beta III Tubulin antibody at 1:5000 dilution.
