ARG62675
anti-beta Endorphin antibody [B31.15]
anti-beta Endorphin antibody [B31.15] for IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Human
Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [B31.15] recognizes beta Endorphin |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-Fr, IHC-P |
| 特异性 | The clone B31.15 reacts with human beta Endorphin, an endogenous opiate derived from ACTH gene. ACTH (Corticotropin; human 39 aa) is synthesized by the anterior pituitary gland and stimulates the adrenal cortex; 6 hormones are derived from one ACTH gene: ACTH, lipotropin, alpha-MSH, beta-MSH, endorphin, and one other. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | B31.15 |
| 同位型 | IgG1 |
| 靶点名称 | beta Endorphin |
| 抗原物种 | Human |
| 抗原 | Human beta Endorphin (full length native protein). |
| 偶联标记 | Un-conjugated |
| 別名 | Alpha-MSH; Beta-MSH; CLIP; Gamma-MSH; LPH; Corticotropin-lipotropin; NPP; ACTH; POMC; Gamma-LPH; Adrenocorticotropic hormone; MSH; Beta-LPH; Pro-opiomelanocortin; POC |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Pretreatment: Heat retrieval (microwave oven). Staining technique: Standard ABC technique (DAB+). Incubation: overnight at 4°C. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||
| 阳性对照 | IHC-P: Human pituitary gland |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | POMC |
| 全名 | proopiomelanocortin |
| 背景介绍 | This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Nov 2014] |
| 生物功能 | ACTH stimulates the adrenal glands to release cortisol. MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin and Met-enkephalin are endogenous opiates. [UniProt] |
| 产品亮点 | Related news: Studying obesity and appetite control by quantifying orexigenic and anorexigenic hormones; |
| 研究领域 | Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 29 kDa |
| 翻译后修饰 | Specific enzymatic cleavages at paired basic residues yield the different active peptides. O-glycosylated; reducing sugar is probably N-acetylgalactosamine. |
检测图片 (1) Click the Picture to Zoom In
-
ARG62675 anti-beta Endorphin antibody [B31.15] IHC-Fr image
Immunohistochemistry: Human pituitary gland (frozen sections) stained with ARG62675 anti-beta Endorphin antibody [B31.15].
克隆号文献
Gonadotropin-inhibitory hormone identification, cDNA cloning, and distribution in rhesus macaque brain.
Detection of mRNA transcripts of truncated opiate precursor (POMC) in human cartilage.
