ARG53987
anti-beta Actin antibody
anti-beta Actin antibody for Western blot and Human,Mouse,Rat,Arabidopsis,Chlamydomonas reinhardtii,Chicken,Cucumber,D. melanogaster ,Fish,Goat,Hamster,Monkey,Pichia pastoris,Pig,Rabbit,Rice,S. cerevisiae,Yeast,Zebrafish
Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Cytochrome-C fractionation Study antibody; Inflammation Study antibody; Tag Internal Control antibody
参考文献2
概述
| 产品描述 | Mouse Monoclonal antibody recognizes beta Actin |
|---|---|
| 反应物种 | Hu, Ms, Rat, Arabi, C. reinhardtii, Chk, Cucumber, Dm, Fsh, Goat, Hm, Mk, P. pastoris, Pig, Rb, Rice, S. cerevisiae, Yeast, Zfsh |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2b |
| 靶点名称 | beta Actin |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human beta actin protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | PS1TP5BP1; BRWS1; Actin, cytoplasmic 1; Beta-actin |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ACTB |
| 全名 | actin, beta |
| 背景介绍 | Beta actin is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017] |
| 生物功能 | Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). [UniProt] |
| 细胞定位 | Cytoplasm, cytoskeleton |
| 产品亮点 | Related Antibody Duos and Panels: ARG30270 Loading Control Antibody Panel (Actin, beta Tublin, Histone H3, GAPDH) ARG30276 Cytochrome-C fractionation Antibody Panel (Cytochrome-C, COX IV, beta Actin) Related products: beta Actin antibodies; beta Actin Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| 研究领域 | Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Cytochrome-C fractionation Study antibody; Inflammation Study antibody; Tag Internal Control antibody |
| 预测分子量 | 42 kDa |
| 翻译后修饰 | ISGylated. Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). |
检测图片 (1) Click the Picture to Zoom In
-
ARG53987 anti-beta Actin antibody WB image
Western blot: 20 µg of HeLa, Mouse brain and Rat brain lysates stained with ARG53987 anti-beta Actin antibody at 1:10000 dilution.
客户反馈
Excellent
anti-beta Actin antibody
Application：WB
Sample：HeLa, Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:10000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-beta Actin antibody
Application：WB
Sample：Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:5000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-beta Actin antibody
Application：WB
Sample：Mouse lung
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:5000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-beta Actin antibody
Application：WB
Sample：Raw
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:5000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-beta Actin antibody
Application：WB
Sample：1) MDAMB231 and 2) MEF
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
Morphofunctional analysis of fibroblast-like synoviocytes in human rheumatoid arthritis and mouse collagen-induced arthritis
WB / Human
Comparative morphofunctional analysis of fibroblast-like synoviocytes in human rheumatoid arthritis and mouse collagen-induced arthritis.
WB / Human
