ARG65693

anti-alpha Tubulin antibody

anti-alpha Tubulin antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Signaling Transduction antibody; Loading Control antibody
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publication_link 参考文献11

概述

产品描述

Rabbit Polyclonal antibody recognizes alpha Tubulin

反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 alpha Tubulin
抗原物种 Human
抗原 Synthetic peptide of Human alpha Tubulin protein.
偶联标记 Un-conjugated
別名 Tubulin K-alpha-1; Alpha-tubulin ubiquitous; Tubulin alpha-ubiquitous chain; Tubulin alpha-1B chain; K-ALPHA-1

应用说明

应用建议
应用 推荐稀释比
FACS1:50 - 1:100
ICC/IF1:200 - 1:500
IHC-P1:200 - 1:500
WB1:5000 - 1:20000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 NIH/3T3, Hela, HepG2, PC12, human stomach tissue, human tonsil tissue, mouse pancreas tissue

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 1*TBS (pH 7.4), 0.05% Sodium azide, 1% BSA and 40% Glycerol
抗菌剂 0.05% Sodium azide
稳定剂 1% BSA, 40% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 10376 Human TUBA1B

GeneID: 22143 Mouse TUBA1B

GeneID: 500929 Rat TUBA1B

基因名称 TUBA1B
全名 tubulin, alpha 1b
生物功能 Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30258 Loading Controls for Whole Cell Lysate Antibody Panel
Related products:
alpha Tubulin antibodies; alpha Tubulin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
研究领域 Signaling Transduction antibody; Loading Control antibody
预测分子量 50 kDa
翻译后修饰 Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).
Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.
Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.
Nitration of Tyr-451 is irreversible and interferes with normal dynein intracellular distribution.
Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
Tubulin alpha-1B chain: Tyrosination promotes microtubule interaction with CAP-Gly domain-containing proteins such as CLIP1, CLIP2 and DCTN1 (By similarity). Tyrosination regulates the initiation of dynein-dynactin motility via interaction with DCTN1, which brings the dynein-dynactin complex into contact with microtubules (PubMed:26972003). In neurons, tyrosinated tubulins mediate the initiation of retrograde vesicle transport (By similarity).
Detyrosinated tubulin alpha-1B chain: Detyrosination is involved in metaphase plate congression by guiding chromosomes during mitosis: detyrosination promotes interaction with CENPE, promoting pole-proximal transport of chromosomes toward the equator (PubMed:25908662). Detyrosination increases microtubules-dependent mechanotransduction in dystrophic cardiac and skeletal muscle. In cardiomyocytes, detyrosinated microtubules are required to resist to contractile compression during contraction: detyrosination promotes association with desmin (DES) at force-generating sarcomeres, leading to buckled microtubules and mechanical resistance to contraction (By similarity).

检测图片 (4) Click the Picture to Zoom In

  • ARG65693 anti-alpha Tubulin antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG65693 anti-alpha Tubulin antibody at 1:200 dilution. Left: primary antibody (orange). Middle: DAPI (blue). Right: Merge.

    Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC)

  • ARG65693 anti-alpha Tubulin antibody WB image

    Western blot: 20 µg of 293T, Mouse brain and Rat brain lysates stained with ARG65693 anti-alpha Tubulin antibody at 1:10000 dilution.

  • ARG65693 anti-alpha Tubulin antibody WB image

    Western blot: 30 µg of HeLa cell lysate stained with ARG65693 anti-alpha Tubulin antibody at 1:5000 dilution.

  • ARG65693 anti-alpha Tubulin antibody WB image

    Western blot: 30 µg of 1) Mouse brain, and 2) Rat brain lysates stained with ARG65693 anti-alpha Tubulin antibody at 1:500 dilution.

客户反馈

nuts_pic      Excellent

anti-alpha Tubulin antibody

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:TRITC

nuts_pic      Excellent

anti-alpha Tubulin antibody

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:TRITC

nuts_pic      Excellent

anti-alpha Tubulin antibody

Application:WB

Sample:293T, Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:10000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-alpha Tubulin antibody

Application:WB

Sample:

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

A novel function and mechanism of ischemia-induced retinal astrocyte-derived exosomes for RGC apoptosis of ischemic retinopathy

WB / Mouse

Xiaoyuan Ye et al.
Molecular Therapy,  (2024)

publication_link

 

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Pharmacokinetic enhancement of oncolytic virus M1 by inhibiting JAK‒STAT pathway

WB / Mouse

Jingyi Tan et al.
Acta Pharmaceutica Sinica B,  (2024)

publication_link

 

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IDH1 mutation impairs antiviral response and potentiates oncolytic virotherapy in glioma

WB / Human, Mouse

Xueqin Chen et al.
Nat Commun.,  (2023)

publication_link

 

hr_line

Sleep deprivation during pregnancy leads to poor fetal outcomes in Sprague-Dawley rats

WB / Rat

Jinzhi Li et al.
J Reprod Immunol.,  (2023)

publication_link

 

hr_line

Targeting VCP potentiates immune checkpoint therapy for colorectal cancer

WB / Mouse

Fang Wang et al.
Cell Rep.,  (2023)

publication_link

 

hr_line

Detection of micro-plasma-induced exosomes secretion in a fibroblast-melanoma co-culture model

WB / Mouse

Han Lee et al.
Analytica Chimica Acta,  (2023)

publication_link

 

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Directly targeting ASC by lonidamine alleviates inflammasome-driven diseases

WB / Mouse

Chen Chen et al.
J Neuroinflammation,  (2022)

publication_link

 

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SOX2 regulates paclitaxel resistance of A549 non‑small cell lung cancer cells via promoting transcription of ClC‑3

WB / Human

Youwei Huang et al.
Oncol Rep,  (2022)

publication_link

 

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Negative regulation of miR-1275 by H3K27me3 is critical for glial induction of glioblastoma cells.

WB / Human WB / Human

Mai J et al.
Mol Oncol.,  (2019)

publication_link

 

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A Novel Synthetic Steroid of 2β,3α,5α-Trihydroxy-androst-6-one Alleviates the Loss of Rat Retinal Ganglion Cells Caused by Acute Intraocular Hypertension via Inhibiting the Inflammatory Activation of Microglia.

WB / Mouse

Sun Hong-Jia-Qi et al.
Molecules.,  (2019)

publication_link

 

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DNA-PK inhibition synergizes with oncolytic virus M1 by inhibiting antiviral response and potentiating DNA damage.

WB / Human

Xiao Xiao et al.
Nat Commun.,  (2018)

publication_link

 

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