ARG43207
anti-alpha Synuclein antibody [2A7]
anti-alpha Synuclein antibody [2A7] for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Cow,Pig
概述
| 产品描述 | Mouse Monoclonal antibody [2A7] recognizes alpha Synuclein |
|---|---|
| 反应物种 | Hu, Ms, Rat, Cow, Pig |
| 应用 | ICC/IF, IHC-Fr, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 2A7 |
| 同位型 | IgG1 |
| 靶点名称 | alpha Synuclein |
| 抗原物种 | Human |
| 抗原 | Recombinant full length Human alpha synuclein protein. |
| 偶联标记 | Un-conjugated |
| 別名 | Non-A4 component of amyloid precursor; Alpha-synuclein; PARK4; PARK1; PD1; NACP; Non-A beta component of AD amyloid |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Rat brain and Rat spinal cord | ||||||||||
| 实际分子量 | ~ 17 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified |
| 缓冲液 | PBS, 5 mM Sodium azide and 50% Glycerol. |
| 抗菌剂 | 5 mM Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SNCA |
| 全名 | synuclein, alpha (non A4 component of amyloid precursor) |
| 背景介绍 | Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016] |
| 生物功能 | Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590). [UniProt] |
| 细胞定位 | Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Note=Membrane-bound in dopaminergic neurons. [UniProt] |
| 预测分子量 | 14 kDa |
| 翻译后修饰 | Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress. Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers. Ubiquitinated. The predominant conjugate is the diubiquitinated form (By similarity). Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG43207 anti-alpha Synuclein antibody [2A7] IHC-Fr image
Immunohistochemistry: Frozen section of Rat cerebellum tissue stained with ARG43207 anti-alpha Synuclein antibody [2A7] (red) at 1:1000 dilution, and costained with anti-GFAP antibody (green) at 1:5000 dilution. Hoechst (blue) for nuclear staining. Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.
The alpha synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells.
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ARG43207 anti-alpha Synuclein antibody [2A7] WB image
Western blot: Rat brain and Rat spinal cord lysates stained with ARG43207 anti-alpha Synuclein antibody [2A7] (green) at 1:1000 dilution.
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ARG43207 anti-alpha Synuclein antibody [2A7] IHC-P image
Immunohistochemistry: Paraffin-embedded cortex tissue of a patient with Parkinson's disease. Tissue section was stained with ARG43207 anti-alpha Synuclein antibody [2A7] at 1:1000 dilution. Antibody revealed with HRP and DAB.
The Lewy bodies and other typical inclusions of Parkinson's disease are seen in brown.
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ARG43207 anti-alpha Synuclein antibody [2A7] IHC-Fr image
Immunohistochemistry: Frozen section of Rat hippocampus tissue stained with ARG43207 anti-alpha Synuclein antibody [2A7] (green) at 1:1000 dilution, and costained with anti-MeCP2 antibody (red) at 1:2000 dilution. DAPI (blue) for nuclear staining. Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45 µM, and free-floating sections were stained with above antibodies.
The alpha synuclein protein is concentrated in synaptic regions, and the MeCP2 antibody stains the nuclei of neuronal cells.
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ARG43207 anti-alpha Synuclein antibody [2A7] IHC-Fr image
Immunohistochemistry: Frozen section of Rat olfactory bulb tissue stained with ARG43207 anti-alpha Synuclein antibody [2A7] (red) at 1:1000 dilution, and costained with anti-GFAP antibody (green) at 1:5000 dilution. DAPI (blue) for nuclear staining. Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.
The alpha synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous backbone of astroglial cells.
