ARG51802
anti-alpha Catenin phospho (Ser641) antibody
anti-alpha Catenin phospho (Ser641) antibody for Western blot and Human,Mouse
Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes alpha Catenin phospho (Ser641) |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | alpha Catenin |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of serine 641 (D-D-S(p)-D-F) derived from Human Catenin alpha. |
| 偶联标记 | Un-conjugated |
| 別名 | Catenin alpha-1; Renal carcinoma antigen NY-REN-13; Cadherin-associated protein; CAP102; Alpha E-catenin |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CTNNA1 |
| 全名 | catenin (cadherin-associated protein), alpha 1, 102kDa |
| 背景介绍 | Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. May play a crucial role in cell differentiation. |
| 生物功能 | Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. [UniProt] |
| 研究领域 | Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody |
| 预测分子量 | 100 kDa |
| 翻译后修饰 | Sumoylated. Phosphorylation seems to contribute to the strength of cell-cell adhesion rather than to the basic capacity for cell-cell adhesion. |
检测图片 (2) Click the Picture to Zoom In
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ARG51802 anti-alpha Catenin phospho (Ser641) antibody WB image
Western blot: Extracts from HeLa cells untreated or treated with starvation stained with ARG51802 anti-alpha Catenin phospho (Ser641) antibody.
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ARG51802 anti-alpha Catenin phospho (Ser641) antibody WB image
Western blot: Extracts from HT29 cells, treated with serum or calf intestinal phosphatase (CIP), stained with ARG51802 anti-alpha Catenin phospho (Ser641) antibody.
