ARG22217

anti-alpha B Crystallin antibody

anti-alpha B Crystallin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Bovine,Chicken

nuts_pic
1

概述

产品描述 Rabbit Polyclonal antibody recognizes alpha B Crystallin
反应物种 Hu, Ms, Rat, Bov, Chk
应用 ICC/IF, IHC-P, WB
特异性 Detects ~22kDa. Does not cross-react with αA-crystallin.
宿主 Rabbit
克隆 Polyclonal
靶点名称 alpha B Crystallin
抗原物种 Human
抗原 KLH-conjugated synthetic peptide from Human alpha B crystallin
偶联标记 Un-conjugated
別名 HSPB5; Rosenthal fiber component; Renal carcinoma antigen NY-REN-27; MFM2; Alpha; CTRCT16; B; HspB5; Heat shock protein beta-5; HEL-S-101; CTPP2; CMD1II; CRYA2; Alpha-crystallin B chain

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:120
IHC-P1:120
WB1:5000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Rabbit antiserum.
缓冲液 Rabbit Antiserum
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 12955 Mouse CRYAB

GeneID: 1410 Human CRYAB

GeneID: 25420 Rat CRYAB

基因名称 CRYAB
全名 crystallin, alpha B
背景介绍 Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
生物功能 May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. [UniProt]
细胞定位 Cytoplasm, Nucleus
预测分子量 20 kDa

检测图片 (4) Click the Picture to Zoom In

  • ARG22217 anti-alpha B Crystallin antibody WB image

    Western blot: 20 µg of U2OS and HeLa cell lysates stained with ARG22217 anti-alpha B Crystallin antibody at 1:1000 dilution.

  • ARG22217 anti-alpha B Crystallin antibody ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22217 anti-alpha B Crystallin antibody (red) at 1:120 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.

  • ARG22217 anti-alpha B Crystallin antibody ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22217 anti-alpha B Crystallin antibody (green) at 1:120 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.

  • ARG22217 anti-alpha B Crystallin antibody WB image

    Western blot: 15 µg of Rat Brain cell lysates stained with ARG22217 anti-alpha B Crystallin antibody at 1:1000 for 60 min at RT. Block: 5% Skim Milk in 1X TBST. Secondary Antibody: Goat anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT.

客户反馈

nuts_pic      Excellent

anti-alpha B Crystallin antibody

Application:WB

Sample:Rat heart

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC