ARG22216
anti-alpha B Crystallin antibody [1A7.D5]
anti-alpha B Crystallin antibody [1A7.D5] for ELISA,ICC/IF,Western blot and Human,Rat,Bovine,Pig
概述
| 产品描述 | Mouse Monoclonal antibody [1A7.D5] recognizes alpha B Crystallin |
|---|---|
| 反应物种 | Hu, Rat, Bov, Pig |
| 应用 | ELISA, ICC/IF, WB |
| 特异性 | Detects ~20kDa (Predicted mol. weight is ~21kDa). Does not cross-react with αA-crystallin, βL-crystallin, βH-crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1A7.D5 |
| 同位型 | IgG1 |
| 靶点名称 | alpha B Crystallin |
| 抗原物种 | Human |
| 抗原 | Native Alpha B Crystallin |
| 偶联标记 | Un-conjugated |
| 別名 | HSPB5; Rosenthal fiber component; Renal carcinoma antigen NY-REN-27; MFM2; Alpha; CTRCT16; B; HspB5; Heat shock protein beta-5; HEL-S-101; CTPP2; CMD1II; CRYA2; Alpha-crystallin B chain |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.2), 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | CRYAB |
| 全名 | crystallin, alpha B |
| 背景介绍 | Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| 生物功能 | May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. [UniProt] |
| 细胞定位 | Cytoplasm, Nucleus |
| 产品亮点 | Related products: anti-alpha B Crystallin antibody [1A7.D5] |
| 预测分子量 | 20 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG22216 anti-alpha B Crystallin antibody [1A7.D5] ICC/IF image
Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary antibody: ARG22216 anti-alpha B Crystallin antibody [1A7.D5] at 1:100 for 60 min at RT. Secondary antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Primary antibody. (D) Composite.
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ARG22216 anti-alpha B Crystallin antibody [1A7.D5] WB image
Western blot: 15 µg of Bovine cell lysates stained with ARG22216 anti-alpha B Crystallin antibody [1A7.D5] at 1:50 dilution (2 hours at RT).
