ARG45408

anti-alpha Adaptin antibody

anti-alpha Adaptin antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes alpha Adaptin
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 alpha Adaptin
抗原物种 Human
抗原 Recombinant protein containing to human alpha Adaptin.
偶联标记 Un-conjugated
別名 AP2A2; Adaptor Related Protein Complex 2 Subunit Alpha 2; HYPJ; HIP9; KIAA0899; CLAPA2; ADTAB; Clathrin-Associated/Assembly/Adaptor Protein, Large, Alpha 2; Clathrin Assembly Protein Complex 2 Alpha-C Large Chain; Plasma Membrane Adaptor HA2/AP2 Adaptin Alpha C Subunit; Alpha-Adaptin C; Huntingtin Interacting Protein J; Adaptor Related Protein Complex 2 Alpha 2 Subunit; 100 KDa Coated Vesicle Protein C; Huntingtin-Interacting Protein 9; AP-2 Complex Subunit Alpha-2; Huntingtin Yeast Partner J; Adaptin, Alpha B; Alpha2-Adaptin; DKFZP564D1864; HIP-9; Adapter-Related Protein Complex 2 Subunit Alpha-2; Adaptor-Related Protein Complex 2 Subunit Alpha-2; Adaptor Protein Complex AP-2 Subunit Alpha-2; Huntingtin-Interacting Protein J; Alpha-Adaptin C

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.1-0.25 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 104 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 11772 Mouse AP2A2

GeneID: 161 Human AP2A2

Swiss-port # O94973 Human AP-2 complex subunit alpha-2

Swiss-port # P17427 Mouse AP-2 complex subunit alpha-2

基因名称 AP2A2
全名 Adaptor Related Protein Complex 2 Subunit Alpha 2
背景介绍 The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane. [provided by RefSeq, Nov 2016]
生物功能 The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. [UniProt]
细胞定位 Cell membrane; Membrane. [UniProt]
预测分子量 104 kDa

检测图片 (6) Click the Picture to Zoom In

  • ARG45408 anti-alpha Adaptin antibody IHC-P image

    Immunohistochemistry: Human lymphoma stained with ARG45408 anti-alpha Adaptin antibody at 2 μg/ml dilution.

  • ARG45408 anti-alpha Adaptin antibody ICC/IF image

    Immunofluorescence: PC-3 stained with ARG45408 anti-alpha Adaptin antibody at 5 μg/ml dilution.

  • ARG45408 anti-alpha Adaptin antibody WB image

    Western blot: HepG2 and HEK293 stained with ARG45408 anti-alpha Adaptin antibody at 0.25 μg/ml dilution.

  • ARG45408 anti-alpha Adaptin antibody FACS image

    Flow Cytometry: 293T stained with ARG45408 anti-alpha Adaptin antibody at 1 µg/10^6 cells dilution.

  • ARG45408 anti-alpha Adaptin antibody WB image

    Western blot: Rat brain stained with ARG45408 anti-alpha Adaptin antibody at 0.25 μg/ml dilution.

  • ARG45408 anti-alpha Adaptin antibody WB image

    Western blot: Mouse brain stained with ARG45408 anti-alpha Adaptin antibody at 0.25 μg/ml dilution.