ARG57053
anti-alpha Actinin antibody [1D10]
anti-alpha Actinin antibody [1D10] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [1D10] recognizes alpha Actinin |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1D10 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | alpha Actinin |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-249 of Human alpha Actinin. |
| 偶联标记 | Un-conjugated |
| 別名 | F-actin cross-linking protein; Non-muscle alpha-actinin-1; BDPLT15; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | ACTN1 |
| 全名 | actinin, alpha 1 |
| 背景介绍 | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. [UniProt] |
| 预测分子量 | 103 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG57053 anti-alpha Actinin antibody [1D10] FACS image
Flow Cytometry: HeLa cell line stained with ARG57053 anti-alpha Actinin antibody [1D10] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57053 anti-alpha Actinin antibody [1D10] WB image
Western blot: 40 µg of 1) PC-3 cell lysate, 2) Balb/3T3 cell lysate, 3) A549 cell lysate stained with ARG57053 anti-alpha Actinin antibody [1D10] at 1:1000.
