ARG40794
anti-alpha 1 Antitrypsin antibody
anti-alpha 1 Antitrypsin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes alpha 1 Antitrypsin |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | alpha 1 Antitrypsin |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 25-315 of Human alpha 1 Antitrypsin (NP_000286.3). |
| 偶联标记 | Un-conjugated |
| 別名 | Serpin A1; alpha1AT; Alpha-1-antiproteinase; Alpha-1 protease inhibitor; AAT; A1A; A1AT; PRO2275; Alpha-1-antitrypsin; PI1; PI; SPAAT |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | SKOV3 | ||||||||
| 实际分子量 | 46 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SERPINA1 |
| 全名 | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 |
| 背景介绍 | The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). [UniProt] |
| 细胞定位 | Secreted. Endoplasmic reticulum. Note=The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum. Short peptide from AAT: Secreted, extracellular space, extracellular matrix. [UniProt] |
| 预测分子量 | 47 kDa |
| 翻译后修饰 | N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG40794 anti-alpha 1 Antitrypsin antibody ICC/IF image
Immunofluorescence: PC-12 cells stained with ARG40794 anti-alpha 1 Antitrypsin antibody at 1:100 dilution.
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ARG40794 anti-alpha 1 Antitrypsin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney tissue stained with ARG40794 anti-alpha 1 Antitrypsin antibody at 1:100 dilution.
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ARG40794 anti-alpha 1 Antitrypsin antibody WB image
Western blot: 25 µg of SKOV3 cell lysate stained with ARG40794 anti-alpha 1 Antitrypsin antibody at 1:1000 dilution.
