ARG55309
anti-alpha 1 / 2 Catenin antibody
anti-alpha 1 / 2 Catenin antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes alpha 1 / 2 Catenin |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | alpha 1 / 2 Catenin |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 637-906 of Human alpha 1 Catenin (NP_001894.2). |
| 偶联标记 | Un-conjugated |
| 別名 | Catenin alpha-1; Renal carcinoma antigen NY-REN-13; Cadherin-associated protein; CAP102; Alpha E-catenin; CAPR; CTNR; CAP-R; CT114 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | 293T and Mouse heart |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CTNNA1, CTNNA2 |
| 全名 | catenin alpha 1, catenin alpha 2 |
| 背景介绍 | This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. [provided by RefSeq, May 2016] |
| 生物功能 | Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. [UniProt] |
| 研究领域 | Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody |
| 预测分子量 | 100 kDa |
| 翻译后修饰 | Sumoylated. Phosphorylation seems to contribute to the strength of cell-cell adhesion rather than to the basic capacity for cell-cell adhesion. |
检测图片 (4) Click the Picture to Zoom In
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ARG55309 anti-alpha 1/2 Catenin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach stained with ARG55309 anti-alpha 1/2 Catenin antibody at 1:100 dilution.
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ARG55309 anti-alpha 1/2 Catenin antibody WB image
Western blot: 25 µg of 293T cell lysate stained with ARG55309 anti-alpha 1/2 Catenin antibody at 1:1000 dilution.
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ARG55309 anti-alpha 1/2 Catenin antibody IP image
Immunoprecipitation: 200 µg extracts of 293T cells were immunoprecipitated and stained with ARG55309 anti-alpha 1/2 Catenin antibody at 1:500 dilution.
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ARG55309 anti-alpha 1/2 Catenin antibody WB image
Western blot: 25 µg of Mouse heart lysate stained with ARG55309 anti-alpha 1/2 Catenin antibody at 1:1000 dilution.
