ARG59889
anti-ZO3 antibody
anti-ZO3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ZO3 |
|---|---|
| 反应物种 | Ms, Rat |
| 预测物种 | Hm |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ZO3 |
| 抗原物种 | Mouse |
| 抗原 | Synthetic peptide corresponding to aa. 885-905 of Mouse ZO3. (RARDVESSDDEGYDWGPATDL) |
| 偶联标记 | Un-conjugated |
| 別名 | ZO3; Tight junction protein ZO-3; Zonula occludens protein 3; Zona occludens protein 3; ZO-3; Tight junction protein 3 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was recommended. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 105 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Thimerosal and 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | TJP3 |
|---|---|
| 全名 | tight junction protein 3 |
| 背景介绍 | The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1. [provided by RefSeq, May 2012] |
| 细胞定位 | Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, tight junction. Nucleus. Note=Exhibits predominant nuclear expression in proliferating cells but is exclusively junctionally expressed after confluence is reached (PubMed:23608536). Shows an epithelial-specific tight junction localization in a TJP1/TJP2-dependent fashion (By similarity). [UniProt] |
| 预测分子量 | 101 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG59889 anti-ZO3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue stained with ARG59889 anti-ZO3 antibody.
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ARG59889 anti-ZO3 antibody WB image
Western blot: Rat brain and Rat heart lysates stained with ARG59889 anti-ZO3 antibody.
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ARG59889 anti-ZO3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse liver tissue stained with ARG59889 anti-ZO3 antibody.
