ARG57552
anti-ZO1 antibody
anti-ZO1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ZO1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ZO1 |
| 抗原物种 | Human |
| 抗原 | Partial recombinant protein around aa. 1178-1527 of Human ZO-1. |
| 偶联标记 | Un-conjugated |
| 別名 | Zonula occludens protein 1; Tight junction protein ZO-1; Tight junction protein 1; Zona occludens protein 1; ZO-1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 194 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.025% Sodium azide and 2.5% BSA. |
| 抗菌剂 | 0.025% Sodium azide |
| 稳定剂 | 2.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TJP1 |
| 全名 | tight junction protein 1 |
| 背景介绍 | This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| 生物功能 | The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. [UniProt] |
| 预测分子量 | 195 kDa |
| 翻译后修饰 | Phosphorylated. Dephosphorylated by PTPRJ. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG57552 anti-ZO1 antibody ICC/IF image
Immunofluorescence: A431 cells stained with ARG57552 anti-ZO1 antibody (green). DAPI (blue) for nuclear staining.
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ARG57552 anti-ZO1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue stained with ARG57552 anti-ZO1 antibody. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).
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ARG57552 anti-ZO1 antibody WB image
Western blot: 1) Rat liver, 2) Mouse liver, 3) Rat NRK, 4) PC12, 5) HeLa, 6) SMMC-7721, 7) Mouse HEPA, 8) COLO320 lysates stained with ARG57552 anti-ZO1 antibody.
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ARG57552 anti-ZO1 antibody FACS image
Flow Cytometry: K562 cells were blocked with goat sera and stained with ARG57552 anti-ZO1 antibody at 1 µg/10^6 cells (blue); Cells alone (red); Isotype control (green).
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ARG57552 anti-ZO1 antibody WB image
Western blot: 0.5 ng of recombinant Human ZO-1 protein stained with ARG57552 anti-ZO1 antibody.
