ARG57121
anti-ZNF9 / CNBP antibody [38F10]
anti-ZNF9 / CNBP antibody [38F10] for Flow cytometry,ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [38F10] recognizes ZNF9 / CNBP |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 38F10 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | ZNF9 / CNBP |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-170 of Human ZNF9 / CNBP |
| 偶联标记 | Un-conjugated |
| 別名 | RNF163; ZCCHC22; PROMM; CNBP1; ZNF9; Zinc finger protein 9; CNBP; DM2; Cellular nucleic acid-binding protein |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P62633 Human Cellular nucleic acid-binding protein |
|---|---|
| 基因名称 | CNBP |
| 全名 | CCHC-type zinc finger, nucleic acid binding protein |
| 背景介绍 | This gene encodes a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion in the first intron of this gene results in myotonic dystrophy type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Single-stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression. [UniProt] |
| 预测分子量 | 19 kDa |
| 翻译后修饰 | Arginine methylation by PRMT1 in the Arg/Gly-rich region impedes RNA binding. |
检测图片 (4) Click the Picture to Zoom In
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ARG57121 anti-ZNF9 / CNBP antibody [38F10] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57121 anti-ZNF9 / CNBP antibody [38F10] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57121 anti-ZNF9 / CNBP antibody [38F10] FACS image
Flow Cytometry: MCF7 cell line stained with ARG57121 anti-ZNF9 / CNBP antibody [38F10] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
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ARG57121 anti-ZNF9 / CNBP antibody [38F10] WB image
Western blot: 30 µg of 293T cell lysate stained with ARG57121 anti-ZNF9 / CNBP antibody [38F10] at 1) 1:250, 2) 1:500, and 3) 1:1000.
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ARG57121 anti-ZNF9 / CNBP antibody [38F10] ICC/IF image
Immunofluorescence: MCF7 cells line stained with ARG57121 anti-ZNF9 / CNBP antibody [38F10] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
