ARG54151
anti-ZAP70 antibody
anti-ZAP70 antibody for Immunoprecipitation,Western blot and Human
Controls and Markers antibody; Immune System antibody; Signaling Transduction antibody; SyK / Zap70 Pathway antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes ZAP70 |
|---|---|
| 反应物种 | Hu |
| 应用 | IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2b |
| 靶点名称 | ZAP70 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human ZAP-70 protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | STD; SRK; STCD; 70 kDa zeta-chain associated protein; Tyrosine-protein kinase ZAP-70; TZK; Syk-related tyrosine kinase; ZAP-70; EC 2.7.10.2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | 70 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | ZAP70 |
| 全名 | zeta-chain (TCR) associated protein kinase 70kDa |
| 背景介绍 | Tyrosine kinase that plays an essential role in regulation of the adaptive immune response.Regulates motility,adhesion and cytokine expression of mature T-cells,as well as thymocyte development.Contributes also to the development and activation of primary B-lymphocytes.When antigen presenting cells (APC) activate T-cell receptor (TCR),a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane.This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation.Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK.Subsequently,ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn,a large number of signaling molecules are recruited and ultimately lead to lymphokine production,T-cell proliferation and differentiation.Furthermore,ZAP70 controls cytoskeleton modifications,adhesion and mobility of T-lymphocytes,thus ensuring correct delivery of effectors to the APC.ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2.Thus,ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface.During thymocyte development,ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative).Additionally,ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR). |
| 生物功能 | Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR). [UniProt] |
| 细胞定位 | Cytoplasm. Cell membrane; Peripheral membrane protein. Note: In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes By similarity. |
| 研究领域 | Controls and Markers antibody; Immune System antibody; Signaling Transduction antibody; SyK / Zap70 Pathway antibody |
| 预测分子量 | 70 kDa |
| 翻译后修饰 | Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Phosphorylation of Tyr-315 and Tyr-319 are essential for ZAP70 positive function on T-lymphocyte activation whereas Tyr-292 has a negative regulatory role. Within the C-terminal kinase domain, Tyr-492 and Tyr-493 are phosphorylated after TCR induction, Tyr-492 playing a negative regulatory role and Tyr-493 a positive. Tyr-493 is dephosphorylated by PTN22. Ubiquitinated in response to T cell activation. Deubiquitinated by OTUD7B. |
检测图片 (2) Click the Picture to Zoom In
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ARG54151 anti-ZAP70 antibody WB image
Western blot: Jurkat cell lysate stained with ARG54151 anti-ZAP70 antibody at 1:1000 dilution.
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ARG54151 anti-ZAP70 antibody IP image
Immunoprecipitation: Jurkat cell lysates were immunoprecipitated and stained with ARG54151 anti-ZAP70 antibody.
