ARG45871

anti-YTHDF1 antibody

anti-YTHDF1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Flow cytometry and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes YTHDF1
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 YTHDF1
抗原物种 Human
抗原 Synthetic peptide corresponding to C-terminal region of human YTHDF1.
偶联标记 Un-conjugated
別名 YTHDF1; YTH N(6)-Methyladenosine RNA Binding Protein 1; DACA-1; Dermatomyositis associated with cancer putative autoantigen 1; C20orf21; YTH domain-containing family protein 1

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 65 kDa

属性

形式 Liquid
纯化 Affinity chromatography purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -22°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 228994 Mouse YTHDF1

GeneID: 54915 Human YTHDF1

Swiss-port # P59326 Mouse YTH domain-containing family protein 1

Swiss-port # Q9BYJ9 Human YTH domain-containing family protein 1

基因名称 YTHDF1
全名 YTH N(6)-Methyladenosine RNA Binding Protein 1
背景介绍 Enables N6-methyladenosine-containing RNA reader activity and ribosome binding activity. Involved in mRNA destabilization; positive regulation of translational initiation; and stress granule assembly. Located in P-body and cytoplasmic stress granule. Implicated in colorectal cancer. [provided by Alliance of Genome Resources, Feb 2025]
生物功能 Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and regulates their stability. [UniProt]
细胞定位 Cytoplasm. [UniProt]
预测分子量 61 kDa
翻译后修饰 Acetylation; Phosphoprotein. [UniProt]

检测图片 (6) Click the Picture to Zoom In

  • ARG45871 anti-YTHDF1 antibody ICC/IF image

    Immunofluorescence: U2OS stained with ARG45871 anti-YTHDF1 antibody at 5 μg/ml dilution.

  • ARG45871 anti-YTHDF1 antibody WB image

    Western blot: A549 stained with ARG45871 anti-YTHDF1 antibody at 1:1000 dilution.

  • ARG45871 anti-YTHDF1 antibody FACS image

    Flow Cytometry: HepG2 stained with ARG45871 anti-YTHDF1 antibody at 1 µg/10^6 cells dilution.

  • ARG45871 anti-YTHDF1 antibody WB image

    Western blot: Rat brain stained with ARG45871 anti-YTHDF1 antibody at 1:1000 dilution.

  • ARG45871 anti-YTHDF1 antibody IHC-P image

    Immunohistochemistry: Mouse testis stained with ARG45871 anti-YTHDF1 antibody at 1:100 dilution.

  • ARG45871 anti-YTHDF1 antibody WB image

    Western blot: Mouse brain stained with ARG45871 anti-YTHDF1 antibody at 1:1000 dilution.