ARG43390
anti-YTHDF1 antibody
anti-YTHDF1 antibody for Immunoprecipitation,Western blot and Human,Hamster
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes YTHDF1 |
|---|---|
| 反应物种 | Hu, Hm |
| 应用 | IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | YTHDF1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human YTHDF1. |
| 偶联标记 | Un-conjugated |
| 別名 | DACA-1; Dermatomyositis associated with cancer putative autoantigen 1; C20orf21; YTH domain-containing family protein 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa | ||||||
| 实际分子量 | ~ 60 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 浓度 | Batch dependent |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9BYJ9 Human YTH domain-containing family protein 1 |
|---|---|
| 基因名称 | YTHDF1 |
| 全名 | YTH N(6)-methyladenosine RNA binding protein 1 |
| 生物功能 | Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency (PubMed:24284625, PubMed:26046440, PubMed:26318451). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:24284625). Acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation (PubMed:26046440). Required to facilitate learning and memory formation in the hippocampus by enhancing protein synthesis upon neuronal stimulation: in response to neuronal stimulation, binds to m6A-containing neuronal mRNAs, promoting their translation, thereby contributing to learning and memory (By similarity). Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts, thereby promoting their translation (By similarity). Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells (By similarity). Acts by binding and promoting translation of m6A-containing transcripts encoding proteins involved in lysosomal degradation and phagosome maturation, leading to increased antigen degradation in myeloid dendritic cells (By similarity). In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 产品亮点 | Related news: m6A reader YTHDF2 in mRNA decay and aggresome formation; |
| 预测分子量 | 61 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG43390 anti-YTHDF1 antibody WB image
Western blot: HeLa cell lysate stained with ARG43390 anti-YTHDF1 antibody at 1:1000 dilution.
