ARG41282
anti-YARS / TyrRS antibody
anti-YARS / TyrRS antibody for Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes YARS / TyrRS |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | YARS / TyrRS |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-300 of Human YARS / TyrRS (NP_003671.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Tyrosyl-tRNA synthetase; CMTDIC; YTS; EC 6.1.1.1; Tyrosine--tRNA ligase, cytoplasmic; TyrRS; TYRRS; YRS |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa | ||||||
| 实际分子量 | 60 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | YARS |
| 全名 | tyrosyl-tRNA synthetase |
| 背景介绍 | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008] |
| 生物功能 | Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 59 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG41282 anti-YARS / TyrRS antibody WB image
Western blot: 25 ug of HeLa cell lysate stained with ARG41282 anti-YARS / TyrRS antibody at 1:1000 dilution.
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ARG41282 anti-YARS / TyrRS antibody IP image
Immunoprecipitation: 200 ug extracts of HeLa cells were immunoprecipitated and stained with ARG41282 anti-YARS / TyrRS antibody at 1:1000 dilution.
