ARG65091
anti-YAP1 antibody
anti-YAP1 antibody for Western blot and Dog
Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes YAP1 |
|---|---|
| 反应物种 | Dog |
| 预测物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | YAP1 |
| 抗原物种 | Human |
| 抗原 | C-TYHSRDESTDS |
| 偶联标记 | Un-conjugated |
| 別名 | YAP65; COB1; Transcriptional coactivator YAP1; Yes-associated protein 1; YKI; YAP; Yes-associated protein YAP65 homolog; Protein yorkie homolog; YAP2 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 背景介绍 | This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq, Jul 2008] |
|---|---|
| 研究领域 | Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation (PubMed:18158288, PubMed:20048001). Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage (PubMed:18280240). Phosphorylation at Ser-400 and Ser-403 by CK1 is triggered by previous phosphorylation at Ser-397 by LATS proteins and leads to YAP1 ubiquitination by SCF(beta-TRCP) E3 ubiquitin ligase and subsequent degradation (PubMed:20048001). Phosphorylated at Thr-119, Ser-138, Thr-154, Ser-367 and Thr-412 by MAPK8/JNK1 and MAPK9/JNK2, which is required for the regulation of apoptosis by YAP1 (PubMed:21364637). Ubiquitinated by SCF(beta-TRCP) E3 ubiquitin ligase. |
检测图片 (1) Click the Picture to Zoom In
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ARG65091 anti-YAP1 antibody WB image
Western Blot: MDCK lysate (35 µg protein in RIPA buffer) stained with ARG65091 anti-YAP1 antibody at 0.5 µg/ml dilution.
