ARG59229
anti-XRCC4 antibody
anti-XRCC4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes XRCC4 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | XRCC4 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 49-75 of Human XRCC4. (ESEISQEADDMAMEKGKYVGELRKALL) |
| 偶联标记 | Un-conjugated |
| 別名 | DNA repair protein XRCC4; SSMED; X-ray repair cross-complementing protein 4 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | XRCC4 |
| 全名 | X-ray repair complementing defective repair in Chinese hamster cells 4 |
| 背景介绍 | The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants. [provided by RefSeq, Sep 2008] |
| 生物功能 | Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 38 kDa |
| 翻译后修饰 | Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX. Monoubiquitinated. Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG59229 anti-XRCC4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer stained with ARG59229 anti-XRCC4 antibody at 1 µg/ml dilution.
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ARG59229 anti-XRCC4 antibody WB image
Western blot: SW620 and A431 whole cell lysates stained with ARG59229 anti-XRCC4 antibody at 0.5 µg/ml dilution.
