ARG43627
anti-XPC antibody
anti-XPC antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes XPC |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | XPC |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to a.a. D146-E838 of Human XPC. |
| 偶联标记 | Un-conjugated |
| Protein Full name | DNA repair protein complementing XP-C cells |
| 別名 | p125; XPCC; Xeroderma pigmentosum group C-complementing protein; RAD4; XP3; DNA repair protein complementing XP-C cells |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | ~120 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.01% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P51612 Mouse DNA repair protein complementing XP-C cells homolog Swiss-port # Q01831 Human DNA repair protein complementing XP-C cells |
|---|---|
| 基因名称 | XPC |
| 全名 | xeroderma pigmentosum, complementation group C |
| 背景介绍 | This gene encodes a component of the nucleotide excision repair (NER) pathway. There are multiple components involved in the NER pathway, including Xeroderma pigmentosum (XP) A-G and V, Cockayne syndrome (CS) A and B, and trichothiodystrophy (TTD) group A, etc. This component, XPC, plays an important role in the early steps of global genome NER, especially in damage recognition, open complex formation, and repair protein complex formation. Mutations in this gene or some other NER components result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009] |
| 生物功能 | Involved in global genome nucleotide excision repair (GG-NER) by acting as damage sensing and DNA-binding factor component of the XPC complex. Has only a low DNA repair activity by itself which is stimulated by RAD23B and RAD23A. Has a preference to bind DNA containing a short single-stranded segment but not to damaged oligonucleotides. This feature is proposed to be related to a dynamic sensor function: XPC can rapidly screen duplex DNA for non-hydrogen-bonded bases by forming a transient nucleoprotein intermediate complex which matures into a stable recognition complex through an intrinsic single-stranded DNA-binding activity. The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1. [UniProt] |
| 细胞定位 | Chromosome; Cytoplasm; Nucleus |
| 预测分子量 | 106 kDa |
| 翻译后修饰 | Ubiquitinated upon UV irradiation; the ubiquitination requires the UV-DDB complex, appears to be reversible and does not serve as a signal for degradation (PubMed:15882621, PubMed:23751493). Ubiquitinated by RNF11 via 'Lys-63'-linked ubiquitination (PubMed:23751493). Ubiquitination by RNF111 is polysumoylation-dependent and promotes nucleotide excision repair (PubMed:23751493). Sumoylated; sumoylation promotes ubiquitination by RNF111. |
检测图片 (5) Click the Picture to Zoom In
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ARG43627 anti-XPC antibody ICC/IF image
Immunofluorescence: HeLa stained with ARG43627 anti-XPC antibody at 5 μg/ml dilution.
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ARG43627 anti-XPC antibody WB image
Western blot: MCF-7 and A549 stained with ARG43627 anti-XPC antibody at 0.5 μg/mL dilution.
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ARG43627 anti-XPC antibody FACS image
Flow Cytometry: A549 stained with ARG43627 anti-XPC antibody at 1 μg / 10^6 cells dilution.
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ARG43627 anti-XPC antibody WB image
Western blot: Rat liver stained with ARG43627 anti-XPC antibody at 0.5 μg/mL dilution.
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ARG43627 anti-XPC antibody WB image
Western blot: Mouse lung stained with ARG43627 anti-XPC antibody at 0.5 μg/mL dilution.
