ARG62661
anti-XPA antibody [12F5]
anti-XPA antibody [12F5] for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody [12F5] recognizes XPA |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-Fr, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 12F5 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | XPA |
| 抗原物种 | Human |
| 抗原 | Recombinant human XPA protein. |
| 偶联标记 | Un-conjugated |
| 別名 | XP1; XPAC; Xeroderma pigmentosum group A-complementing protein; DNA repair protein complementing XP-A cells |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Ls174T or MCF-7 cells or Human tonsil. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified Antibody |
| 缓冲液 | 1X PBS and 0.1% Sodium azide |
| 抗菌剂 | 0.1% Sodium azide |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P23025 Human DNA repair protein complementing XP-A cells |
|---|---|
| 基因名称 | XPA |
| 全名 | xeroderma pigmentosum, complementation group A |
| 背景介绍 | This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009] |
| 生物功能 | Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. [UniProt] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 31 kDa |
| 翻译后修饰 | ATR-dependent phosphorylation of XPA at Ser-196 is important for cell survival in response to UV damage. Ubiquitinated by HERC2 leading to degradation by the proteasome. |
克隆号文献
Nucleotide excision repair is not induced in human embryonic lung fibroblasts treated with environmental pollutants.
WB /
Nucleotide excision repair is associated with the replisome and its efficiency depends on a direct interaction between XPA and PCNA.
ICC/IF / Human
Poly(ADP-ribose) contributes to an association between poly(ADP-ribose) polymerase-1 and xeroderma pigmentosum complementation group A in nucleotide excision repair.
WB, ICC/IF / Human
Crosslinking of DNA repair and replication proteins to DNA in cells treated with 6-thioguanine and UVA.
WB / Human
Recognition of cisplatin-DNA interstrand cross-links by replication protein A.
WB / Bovine
DNA polymerase epsilon associates with the elongating form of RNA polymerase II and nascent transcripts.
IP / Human
Expression of xeroderma pigmentosum A protein predicts improved outcome in metastatic ovarian carcinoma.
IHC / Human
