ARG41408
anti-Wnt10b antibody
anti-Wnt10b antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Wnt10b |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | IHC-P, WB |
| 特异性 | Two isoforms of Wnt10b are known to exist; this antibody will only recognize the longer isoform. This Wnt10b antibody will not cross-react with Wnt10a. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Wnt10b |
| 抗原物种 | Human |
| 抗原 | A 15 amino acid synthetic peptide around the center region of Human Wnt10b. |
| 偶联标记 | Un-conjugated |
| 別名 | WNT-12; Protein Wnt-12; SHFM6; Protein Wnt-10b |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Human skeletal muscle | ||||||
| 实际分子量 | ~ 47 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | WNT10B |
| 全名 | wingless-type MMTV integration site family, member 10B |
| 背景介绍 | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008] |
| 生物功能 | Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). [UniProt] |
| 细胞定位 | Secreted, extracellular space, extracellular matrix. Secreted. [UniProt] |
| 预测分子量 | 43 kDa |
| 翻译后修饰 | Palmitoleylation is required for efficient binding to frizzled receptors. Depalmitoleylation leads to Wnt signaling pathway inhibition. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG41408 anti-Wnt10b antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue stained with ARG41408 anti-Wnt10b antibody at 2.5 µg/ml dilution.
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ARG41408 anti-Wnt10b antibody WB image
Western blot: Human skeletal muscle lysate stained with ARG41408 anti-Wnt10b antibody at 2 and 4 µg/ml dilution.
