ARG55020
anti-WWTR1 / TAZ antibody
anti-WWTR1 / TAZ antibody for Western blot and Human,Mouse
Developmental Biology antibody; Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes WWTR1 / TAZ |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | WWTR1 / TAZ |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 296-330 (C-terminus) of Human WWTR1. |
| 偶联标记 | Un-conjugated |
| 別名 | Transcriptional coactivator with PDZ-binding motif; WW domain-containing transcription regulator protein 1; TAZ |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | HeLa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9EPK5 Mouse WW domain-containing transcription regulator protein 1 Swiss-port # Q9GZV5 Human WW domain-containing transcription regulator protein 1 |
|---|---|
| 基因名称 | WWTR1 |
| 全名 | WW domain containing transcription regulator 1 |
| 生物功能 | Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition. [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. Note=Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies. When phosphorylated, is retained in cytoplasm by YWHAZ. Can be retained in the nucleus by MED15 |
| 研究领域 | Developmental Biology antibody; Gene Regulation antibody |
| 预测分子量 | 44 kDa |
| 翻译后修饰 | Phosphorylated by LATS2 and STK3/MST2. Phosphorylation by LATS2 results in creation of 14-3-3 binding sites, retention in the cytoplasm, and functional inactivation. Phosphorylation results in the inhibition of transcriptional coactivation through YWHAZ-mediated nuclear export. |
检测图片 (1) Click the Picture to Zoom In
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ARG55020 anti-WWTR1 / TAZ antibody WB image
Western blot: 20 µg of HeLa cell lysate stained with ARG55020 anti-WWTR1 / TAZ antibody at 1:1000 dilution.
