ARG42667
anti-WWP1 antibody
anti-WWP1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes WWP1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | WWP1 |
| 抗原物种 | Human |
| 抗原 | Fusion protein of Human WWP1. |
| 偶联标记 | Un-conjugated |
| 別名 | NEDD4-like E3 ubiquitin-protein ligase WWP1; WW domain-containing protein 1; EC 6.3.2.-; Atrophin-1-interacting protein 5; Tiul1; hSDRP1; AIP5; TGIF-interacting ubiquitin ligase 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | WB: MDA-MB-231 and HeLa cell lysates IHC-P: Human esophagus cancer tissue |
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| 实际分子量 | ~ 110 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 40% Glycerol |
| 浓度 | 0.4 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9H0M0 Human NEDD4-like E3 ubiquitin-protein ligase WWP1 |
|---|---|
| 基因名称 | WWP1 |
| 全名 | WW domain containing E3 ubiquitin protein ligase 1 |
| 背景介绍 | WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined. [provided by RefSeq, Jul 2008] |
| 生物功能 | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF. [UniProt] |
| 细胞定位 | Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. [UniProt] |
| 预测分子量 | 105 kDa |
| 翻译后修饰 | Auto-ubiquitinated and ubiquitinated by RNF11. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG42667 anti-WWP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human esophagus cancer tissue stained with ARG42667 anti-WWP1 antibody (left) at 1:40 dilution, or the same antibody pre-incubated with fusion protein (right). (Original magnification: X200)
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ARG42667 anti-WWP1 antibody WB image
Western blot: 40 µg of MDA-MB-231 and HeLa cell lysates stained with ARG42667 anti-WWP1 antibody at 1:500 dilution.
