ARG59158
anti-WRN antibody
anti-WRN antibody for Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes WRN |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | WRN |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1223-1432 of Human WRN (NP_000544.2). |
| 偶联标记 | Un-conjugated |
| 別名 | Exonuclease WRN; RecQ3; Werner syndrome ATP-dependent helicase; RECQL3; RECQL2; RECQ3; EC 3.1.-.-; RecQ protein-like 2; EC 3.6.4.12; DNA helicase, RecQ-like type 3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | K562 | ||||
| 实际分子量 | 200 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q14191 Human Werner syndrome ATP-dependent helicase |
|---|---|
| 基因名称 | WRN |
| 全名 | Werner syndrome, RecQ helicase-like |
| 背景介绍 | This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging. [provided by RefSeq, Jul 2008] |
| 生物功能 | Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation. [UniProt] |
| 细胞定位 | Nucleus, nucleolus. Nucleus. Nucleus, nucleoplasm. Note=Gamma-irradiation leads to its translocation from nucleoli to nucleoplasm and PML regulates the irradiation-induced WRN relocation. [UniProt] |
| 预测分子量 | 162 kDa |
| 翻译后修饰 | Phosphorylated by PRKDC. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG59158 anti-WRN antibody WB image
Western blot: 25 µg of K562 cell lysate stained with ARG59158 anti-WRN antibody at 1:1000 dilution.
