ARG66415
anti-WNK1 phospho (Thr60) antibody
anti-WNK1 phospho (Thr60) antibody for Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes WNK1 phospho (Thr60) |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 特异性 | The antibody detects endogenous levels of WNK1 protein only when phosphorylated at Thr60. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | WNK1 |
| 抗原物种 | Human |
| 抗原 | Phosphospecific peptide around Thr60 of Human WNK1. |
| 偶联标记 | Un-conjugated |
| 別名 | PSK; p65; PRKWNK1; Serine/threonine-protein kinase WNK1; Protein kinase lysine-deficient 1; PPP1R167; Erythrocyte 65 kDa protein; hWNK1; Kinase deficient protein; EC 2.7.11.1; HSN2; KDP; HSAN2; Protein kinase with no lysine 1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9H4A3 Human Serine/threonine-protein kinase WNK1 |
|---|---|
| 基因名称 | WNK1 |
| 全名 | WNK lysine deficient protein kinase 1 |
| 背景介绍 | This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010] |
| 生物功能 | Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 251 kDa |
| 翻译后修饰 | O-glycosylated. Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG66415 anti-WNK1 phospho (Thr60) antibody WB image
Western blot: 293 cells stained with ARG66415 anti-WNK1 phospho (Thr60) antibody.
