ARG41764
anti-WFDC2 / HE4 antibody
anti-WFDC2 / HE4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes WFDC2 / HE4 |
|---|---|
| 反应物种 | Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | WFDC2 / HE4 |
| 抗原物种 | Rat |
| 抗原 | Recombinant protein corresponding to E31-F168 of Rat WFDC2 / HE4. |
| 偶联标记 | Un-conjugated |
| 別名 | HE4; dJ461P17.6; WAP5; Putative protease inhibitor WAP5; Major epididymis-specific protein E4; WAP four-disulfide core domain protein 2; Epididymal secretory protein E4; EDDM4 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 18 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q8CHN3 Rat WAP four-disulfide core domain protein 2 Swiss-port # Q9DAU7 Mouse WAP four-disulfide core domain protein 2 |
|---|---|
| 基因名称 | WFDC2 |
| 全名 | WAP four-disulfide core domain 2 |
| 背景介绍 | This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008] |
| 生物功能 | Broad range protease inhibitor. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 预测分子量 | 13 kDa |
检测图片 (6) Click the Picture to Zoom In
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ARG41764 anti-WFDC2 / HE4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse lung tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41764 anti-WFDC2 / HE4 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41764 anti-WFDC2 / HE4 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat testis, Rat brain, Rat thymus, Mouse testis, Mouse brain, Mouse thymus and HEPA1-6 whole cell lysates stained with ARG41764 anti-WFDC2 / HE4 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG41764 anti-WFDC2 / HE4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse small intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41764 anti-WFDC2 / HE4 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41764 anti-WFDC2 / HE4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41764 anti-WFDC2 / HE4 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41764 anti-WFDC2 / HE4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat lung tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41764 anti-WFDC2 / HE4 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41764 anti-WFDC2 / HE4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat small intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41764 anti-WFDC2 / HE4 antibody at 1 µg/ml dilution, overnight at 4°C.
