ARG55245

anti-WEE1 antibody

anti-WEE1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Gene Regulation antibody

概述

产品描述 Rabbit Polyclonal antibody recognizes WEE1
反应物种 Hu, Ms, Rat
应用 IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 WEE1
抗原物种 Human
抗原 KLH-conjugated synthetic peptide corresponding to aa. 144-173 (Center) of Human WEE1.
偶联标记 Un-conjugated
別名 Wee1A kinase; Wee1-like protein kinase; WEE1hu; WEE1A; EC 2.7.10.2

应用说明

应用建议
应用 推荐稀释比
IHC-P1:50 - 1:100
WB1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS and 0.09% (W/V) Sodium azide
抗菌剂 0.09% (W/V) Sodium azide
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 22390 Mouse WEE1

GeneID: 308937 Rat WEE1

GeneID: 7465 Human WEE1

基因名称 WEE1
全名 WEE1 G2 checkpoint kinase
背景介绍 This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]
生物功能 Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. [UniProt]
细胞定位 Nucleus.
研究领域 Cell Biology and Cellular Response antibody; Gene Regulation antibody
预测分子量 72 kDa
翻译后修饰 Phosphorylated during M and G1 phases. Also autophosphorylated. Phosphorylation at Ser-642 by BRSK1 and BRSK2 in post-mitotic neurons, leads to down-regulate WEE1 activity in polarized neurons. Phosphorylated at Ser-53 and Ser-123 by PLK1 and CDK1, respectively, generating an signal for degradation that can be recognized by the SCF(BTRC) complex, leading to its ubiquitination and degradation at the onset of G2/M phase.
Dephosphorylated at Thr-239 by CTDP1.
Ubiquitinated and degraded at the onset of G2/M phase.

检测图片 (2) Click the Picture to Zoom In

  • ARG55245 anti-WEE1 antibody WB image

    Western blot: 30 μg of HeLa, Jurkat, Mouse heart and Rat heart lysates stained with ARG55245 anti-WEE1 antibody at 1:500 dilution.

  • ARG55245 anti-WEE1 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human cancer tissue stained with ARG55245 anti-WEE1 antibody.