ARG54144
anti-WDR77 antibody
anti-WDR77 antibody for ICC/IF,Western blot and Human,Mouse,Rat
Gene Regulation antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes MEP50/WDR77 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG1 |
| 靶点名称 | WDR77 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human WDR77 protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | Nbla10071; p44/Mep50; WD repeat-containing protein 77; MEP50; HKMT1069; Methylosome protein 50; p44; MEP-50; Androgen receptor cofactor p44 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | 42 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | WDR77 |
| 全名 | WD repeat domain 77 |
| 背景介绍 | Non-catalytic component of the 20S PRMT5-containing methyltransferase complex,which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones.This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles.Might play a role in transcription regulation.The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4),methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. |
| 生物功能 | Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. |
| 研究领域 | Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 37 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG54144 anti-WDR77 antibody ICC/IF image
Immunofluorescence: HeLa cells fixed in 1% Paraformaldehyde, permeabilized in 0.1% Triton X-100 and stained with ARG54144 anti-WDR77 antibody at 1:100 dilution.
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ARG54144 anti-WDR77 antibody WB image
Western blot: K562 cell lysate stained with ARG54144 anti-WDR77 antibody at 1:1000 dilution.
