ARG63714
anti-WASP / Wiskott Aldrich Syndrome antibody
anti-WASP / Wiskott Aldrich Syndrome antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human
Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes WASP / Wiskott Aldrich Syndrome |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Dog |
| 应用 | FACS, ICC/IF, IHC-P |
| 特异性 | No cross-reactivity expected with N WASP (WASL). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | WASP / Wiskott Aldrich Syndrome |
| 抗原物种 | Human |
| 抗原 | C-SPADKKRSGKKKI |
| 偶联标记 | Un-conjugated |
| 別名 | WASp; WASPA; IMD2; THC1; THC; WASP; SCNX; Wiskott-Aldrich syndrome protein |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq, Jul 2008] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 53 kDa |
| 翻译后修饰 | Phosphorylated at Tyr-291 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-291 enhances WAS activity in promoting actin polymerization and filopodia formation. |
检测图片 (4) Click the Picture to Zoom In
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ARG63714 anti-WASP / Wiskott Aldrich Syndrome antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HepG2 cells permeabilized with 0.15% Triton. Cells were stained with ARG63714 anti-WASP / Wiskott Aldrich Syndrome antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63714 anti-WASP / Wiskott Aldrich Syndrome antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63714 anti-WASP / Wiskott Aldrich Syndrome antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63714 anti-WASP / Wiskott Aldrich Syndrome antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HepG2 cells permeabilized with 0.5% Triton. Cells were stained with ARG63714 anti-WASP / Wiskott Aldrich Syndrome antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG63714 anti-WASP / Wiskott Aldrich Syndrome antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63714 anti-WASP / Wiskott Aldrich Syndrome antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
